Hemoglobin Subunit Epsilon Antibody (PrecisionAbTM Antibody)

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Hemoglobin Subunit Epsilon Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with hemoglobin subunit epsilon antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 77 second exposure. Arrow points to hemoglobin subunit epsilon (molecular weight 14 kDa)

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  • Rabbit anti Hemoglobin Subunit Epsilon
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    Rabbit anti-human hemoglobin subunit epsilon antibody recognizes hemoglobin subunit epsilon, also known as epsilon globin. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00539WBdatasheet pdfdatasheet pdf100 µl
      VPA00539
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human hemoglobin subunit epsilon antibody recognizes hemoglobin subunit epsilon also known as epsilon globin.

        The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' (provided by RefSeq, Jul 2008).

        Rabbit anti Human hemoglobin subunit epsilon antibody detects a band of 14 kDa. The antibody has been extensively validated for western blotting using tissue and whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti hemoglobin subunit epsilon detects a band of approximately 14 kDa in mouse liver
      • Product Form
        Purified IgG - liquid
      • Preparation
        Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH conjugated synthetic peptide corresponding to amino acid 55-83 of human hemoglobin subunit epsilon
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        oxygen binding
        blood coagulation
        hemoglobin complex
        heme binding
        oxygen transporter activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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