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Heat Shock Protein 90 Alpha antibody

Rabbit anti Heat Shock Protein 90 Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Heat Shock Protein 90 Alpha

Product Code Applications Pack Size List Price Your Price Qty
AHP2473
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF P WB 50 µl loader
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loader

Rabbit anti heat shock protein 90 alpha antibody recognizes heat shock protein 90 alpha also known as HSP90AA1 or LAP2. Heat shock protein 90 alpha is an isoform of the highly conserved heat shock protein 90. Heat shock protein 90 alpha expression is induced under conditions of cellular stress where upon it acts as a molecular chaperone.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to heat shock protein 90 alpha was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% ProClin 300
50% Glycerol
Immunogen
Recombinant human heat shock protein 90 alpha
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/20 1/50
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti heat shock protein 90 alpha antibody detects a band of approximately 100 kDa in cell lysates and tissue lysates under reducing conditions

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References for Heat Shock Protein 90 Alpha antibody

  1. Chen, C.C. et al. (2018) Myeloid-derived macrophages and secreted HSP90α induce pancreatic ductal adenocarcinoma development.
    Oncoimmunology. 7 (5): e1424612.

Western Blotting

UniProt
P07900
Entrez Gene
HSP90AA1
GO Terms
GO:0051082 unfolded protein binding
GO:0005886 plasma membrane
GO:0000086 G2/M transition of mitotic cell cycle
GO:0006986 response to unfolded protein
GO:0042026 protein refolding
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005524 ATP binding
GO:0007411 axon guidance
GO:0030235 nitric-oxide synthase regulator activity
GO:0030911 TPR domain binding
GO:0034619 cellular chaperone-mediated protein complex assembly
GO:0042470 melanosome
GO:0042803 protein homodimerization activity
GO:0045040 protein import into mitochondrial outer membrane
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0046209 nitric oxide metabolic process
GO:0050999 regulation of nitric-oxide synthase activity

AHP2473

154698 165055

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