Heat Shock Protein 75 Antibody (PrecisionAbTM Antibody) | 1A1

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Heat Shock Protein 75 Antibody (PrecisionAb<sup>TM</sup> Antibody) | 1A1 gallery image 1

Western blot analysis of whole cell lysates probed with heat shock protein 75 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to heat shock protein 75 (molecular weight 75 kDa)

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  • Heat Shock Protein 75 Antibody (PrecisionAb<sup>TM</sup> Antibody) | 1A1 thumbnail image 1
  • Mouse anti Heat Shock Protein 75
(Rated 0.0 out of 5 based on 0 customer reviews)
    Mouse anti-human TRAP1 antibody recognizes heat shock protein 75 also known as TNFR-associated protein 1 or tumor necrosis factor type 1 receptor-associated protein. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00362WBdatasheet pdfdatasheet pdf100 µl
      VMA00362
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human heat shock protein 75 antibody recognizes heat shock protein 75 kDa, mitochondrial, also known as TNFR-associated protein 1, TRAF1 and tumor necrosis factor type 1 receptor-associated protein.

        Encoded by the TRAP1 gene, heat shock protein 75 is a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. Heat shock protein 75 has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants (provided by RefSeq, Jan 2013).

        Mouse anti Human heat shock protein 75 antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti heat shock protein 75 detects a band of approximately 75 kDa in HCT-116 cell lysates
      • Product Form
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography from ascites
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length recombinant human heat shock protein 75 (NP_057376) produced in HEK293T cells
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        unfolded protein binding
        protein folding
        cellular response to oxidative stress
        ATP binding
        tumor necrosis factor receptor binding
        mitochondrion
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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