Heat Shock Protein 70 Antibody | 4E7

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Heat Shock Protein 70 Antibody | 4E7 gallery image 1

Formalin fixed, paraffin embedded human eccrine gland stained with Mouse anti Human heat shock protein 70 (MCA4627GA) following heat mediated antigen retrieval in 0.1M citrate buffer pH6.0

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Heat Shock Protein 70 Antibody | 4E7 gallery image 2

Western blot analysis of heat shock protein in a HeLa cell lysate probed with Mouse anti Human heat shock protein 70 (MCA4627GA)

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  • Heat Shock Protein 70 Antibody | 4E7 thumbnail image 1
  • Heat Shock Protein 70 Antibody | 4E7 thumbnail image 2
  • Mouse anti Human Heat Shock Protein 70
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  • Product Type
    Monoclonal Antibody
  • Clone
    4E7
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4627GAE, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4627GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human heat shock protein 70 antibody, clone 4E7 recognises human heat shock protein 70 (HSP70), a member of the highly related HSP70 family, which range in size from 66-78kDa.

      Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP70 acts alongside other chaperones to mediate the folding of newly-synthesized peptide sequences, stabilize proteins against aggregation, and assist in protein transmembrane transport.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/5001/2000
      Immunohistology - Paraffin(1)1/1001/200
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Eccrine glands in Human skin.
    • Immunofluorescence
    • Western Blotting
      MCA4627GA detects a band of approximately 70 kDa in HeLa cell lysates.
    • Instructions For Use

    Additional Heat Shock Protein 70 Antibody Formats

    Formats Clone Applications Sizes available
    Heat Shock Protein 70 Antibody : Purified 4E7 E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Eccrine glands in Human skin.

              References

              Further Reading

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