Heat Shock Protein 60 Antibody (PrecisionAbTM Antibody)

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Heat Shock Protein 60 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with heat shock protein 60 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to heat shock protein 60 (molecular weight 60 kDa)

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  • Heat Shock Protein 60 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Heat Shock Protein 60 Antibody with Control Lysate
  • Mouse anti Heat Shock Protein 60
  • Heat Shock Protein 60 Antibody with Control Lysate
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    Heat shock protein 60 antibody recognizes mitochondrial 60 kDa heat shock protein, also known as 60 kDa chaperonin, chaperonin 60, CPN60 heat shock protein 60, HSP-60, Hsp60 HuCHA60 Mitochondrial matrix protein, or P1 P60 lymphocyte protein. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00041WBdatasheet pdfdatasheet pdf100 µl
      VMA00041
      VMA00041KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00041K
      VMA00041KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00041KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human heat shock protein 60 antibody recognizes human heat shock protein 60 (HSP60), a member of the highly conserved chaperonin (HSP60) family, expressed abundantly in the mitochondrial matrix and is closely related to E. coli GroEL and plant Rubisco binding protein chaperones.

        Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP60 acts in conjunction with the co-chaperonin HSP10, and is important in the refolding and transportation of proteins from the cytoplasm into the mitochondrial matrix, and also involved in the replication of mitochondrial DNA (Höhfeld et al. 1994).

        Mouse anti Human heat shock protein 60 recognizes human HSP60 as a single band of ~60 kDa by western blotting under reducing conditions in multiple cell line lysates.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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