Heat Shock 70 Kda Protein 5 Antibody (PrecisionAbTM Antibody)

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Heat Shock 70 Kda Protein 5 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with heat shock 70 kDa protein 5 antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to heat shock 70 kDa protein 5 (molecular weight 78 kDa)

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  • Heat Shock 70 Kda Protein 5 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Heat Shock 70 Kda Protein 5 Antibody with Control Lysate
  • Heat Shock 70 Kda Protein 5 Antibody with Control Lysate
  • Human anti Heat Shock 70 Kda Protein 5
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    Anti human heat shock 70 kDa protein 5 antibody recognizes HSPA5, also known as glucose regulated protein, 78 kDa, GRP78, BIP or epididymis secretory sperm binding protein Li 89n. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      HuCAL Fab bivalent
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00443WBdatasheet pdfdatasheet pdf100 µl
      VMA00443
      VMA00443KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00443K
      VMA00443KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00443KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Human anti Human heat shock protein 5 antibody recognizes heat shock protein 5 also known as 78 kDa glucose-regulated protein, GRP78, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, BIP, epididymis secretory sperm binding protein Li 89n, immunoglobulin heavy chain-binding protein and MIF2.

        Encoded by the HSPA5 gene and a member of the heat shock protein 70 (HSP70) family, heat shock protein 5 is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).

        Human anti Human heat shock protein 5 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti HSPA5 detects a band of approximately 78 kDa in HepG2 cell lysates
        Anti HSPA5 detects a band of approximately 78 kDa in HepG2 cell lysates
        Anti heat shock 70 kDa protein 5 detects a band of approximately 78 kDa in HepG2 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Preparation
        Pack Size: 10 Westerns100μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
        Pack Size: 2 Westerns20μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
        Pack Size: 10 Westerns100μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
        Pack Size: 2 Westerns20μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
        Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant human heat Shock 70 kDa protein 5
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HepG2 lysate lyophilized in RIPA buffer
        400μg HepG2 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        endoplasmic reticulum lumen
        negative regulation of caspase activity
        calcium ion binding
        unfolded protein binding
        protein binding, bridging
        melanosome
        platelet activation
        perinuclear region of cytoplasm
        cell surface
        platelet degranulation
        ER-Golgi intermediate compartment
        ATP binding
        ER-associated protein catabolic process
        midbody
        chaperone binding
        regulation of protein folding in endoplasmic reticulum
        anti-apoptosis
        integral to endoplasmic reticulum membrane
        ubiquitin protein ligase binding
        caspase inhibitor activity
        cellular response to glucose starvation
        nucleus
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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