Heat Shock 70 Kda Protein 5 Antibody (PrecisionAbTM Antibody) | CD01/1A7

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Heat Shock 70 Kda Protein 5 Antibody (PrecisionAb<sup>TM</sup> Antibody) | CD01/1A7 gallery image 1

Western blot analysis of whole cell lysates probed with heat shock 70 kDa protein 5 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to heat shock 70 kDa protein 5 (molecular weight 78 kDa)

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  • Heat Shock 70 Kda Protein 5 Antibody (PrecisionAb<sup>TM</sup> Antibody) | CD01/1A7 thumbnail image 1
  • Heat Shock 70 Kda Protein 5 Antibody with Control Lysate
  • Mouse anti Heat Shock 70 Kda Protein 5
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  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG1
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00570WBdatasheet pdfdatasheet pdf100 µl
    VMA00570
    VMA00570KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00570KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
    -
    • Mouse anti Human heat shock 70 kDa protein 5 antibody recognizes heat shock 70 kDa protein 5 also known as GRP78, HSPA5 or 78 kDa glucose-regulated protein.

      Heat shock 70 kDa protein 5 is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).

      Mouse anti Human heat shock 70 kDa Protein 5 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Anti heat shock 70 kDa protein 5 detects a band of approximately 78 kDa in HeLa cell lysates
      Anti heat shock 70 kDa protein 5 detects a band of approximately 78 kDa in HeLa cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      E. coli-derived recombinant human heat shock 70 kDa protein 5 (amino acids 1 - 654)
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg HeLa lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      cell surface
      ER-Golgi intermediate compartment
      anti-apoptosis
      perinuclear region of cytoplasm
      midbody
      calcium ion binding
      integral to endoplasmic reticulum membrane
      ATP binding
      caspase inhibitor activity
      regulation of protein folding in endoplasmic reticulum
      protein binding, bridging
      endoplasmic reticulum lumen
      platelet degranulation
      platelet activation
      cellular response to glucose starvation
      chaperone binding
      ubiquitin protein ligase binding
      unfolded protein binding
      ER-associated protein catabolic process
      negative regulation of caspase activity
      nucleus
      melanosome
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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