Haptoglobin Antibody (PrecisionAbTM Antibody)

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Haptoglobin Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell, human liver and human plasma lysates probed with Haptoglobin antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to Haptoglobin (molecular weight 43 kDa)

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  • Mouse anti Haptoglobin
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    Haptoglobin antibody recognizes the haptoglobin protein, also known as haptoglobin alpha (1S)-beta, haptoglobin alpha (2FS)-beta, liver regeneration-related protein LRRG173 or zonulin. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00089WBdatasheet pdfdatasheet pdf100 µl
      VMA00089
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human haptoglobin antibody recognizes haptoglobin, one of the major acute phase plasma proteins (APP).

        The haptoglobin gene (HP) encodes a preproprotein, also known as zonulin, which is processed to yield both alpha and beta chains. These chains subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin.

        Mutations in HP and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. HP has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to HP on chromosome 16. Multiple transcript variants encoding different isoforms have been found for HP (provided by RefSeq, Jul 2008).

        Mouse anti Human haptoglobin antibody detects a band of 43 kDa. The antibody has been extensively validated for western blotting using human plasma lysates.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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