Haptoglobin Antibody

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  • Sheep anti Human Haptoglobin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP082IDdatasheet pdfdatasheet pdf1 ml
    AHP082
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Sheep anti Human haptoglobin antibody recognizes human haptoglobulin, a plasma protein that has high affinity for hemoglobin and is responsible for its capture and hepatic recycling. This process also acts to prevent kidney damage where hemoglobin would otherwise accumulate prior to excretion in the urine. Haptoglobin/hemoglobin complexes are cleared from the circulation in the liver by Kuppfer cells expressing the CD163 scavanger receptor.

      Two isoforms of human haptoglobin produced by alternative splicing have been described. Sheep anti Human haptoglobin antibody (AHP082) binds to the canonical isoform 1 of human haptoglobin, binding to the truncated (missing residues 38-96) isoform 2 has not been evaluated.

      Mutation of the HP gene can lead to the development of Anhaptoglobinemia (AHP), a disease characterized by an absence of serum haptoglobin (Teye et al. 2004).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Catyes
      Rabbityes
      Mouseyes
      Bovineno
      Dogyes
      Chickenno
      Horseyes
      Guinea Pigno
      Pigyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human haptoglobulin were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.1%EACA
      0.01%Benzamidine
      1mMEDTA
    • Immunogen
      Human haptoglobulin purified from serum.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Glycine buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      positive regulation of cell death
      serine-type endopeptidase activity
      negative regulation of hydrogen peroxide catabolic process
      extracellular region
      negative regulation of oxidoreductase activity
      cellular iron ion homeostasis
      hemoglobin binding
      proteolysis
      defense response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ImmunodiffusionNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Haptoglobin Antibody Formats

    Formats Applications Sizes available
    Haptoglobin Antibody : Purified ID 1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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