H-Ras Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-h-ras-antibody-vma00040.html?RenderPageSpecific=1
H-Ras Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with H-Ras antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 124 second exposure. Arrow points to H-Ras (molecular weight 21 kDa)

Enlarge
H-Ras Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 2

Western blot analysis of whole cell lysates probed with H-Ras antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 300 second exposure. Arrow points to H-Ras (molecular weight 21 kDa)

Enlarge
  • H-Ras Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • H-Ras Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 2
  • H-Ras Antibody with Lysate Control
  • Mouse anti H-Ras
(Rated 0.0 out of 5 based on 0 customer reviews)
    H-Ras antibody recognizes GTPase HRas, also known as H-Ras-1, Ha-Ras, transforming protein p21, c-H-ras or p21ras. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00040WBdatasheet pdfdatasheet pdf100 µl
      VMA00040
      VMA00040KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00040KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human H-Ras antibody recognizes the human GTPase H-Ras also known as H-Ras, transforming protein p21 or p21ras. H-Ras is a ubiquitously expressed lipid-anchored GTPase belonging to the Ras superfamily of small GTPases, which acts as a molecular signal transduction on/off switch on the inner surface of the plasma membrane and endomembranes (Milburn et al. 1990).

        H-Ras, N-Ras and K-Ras are highly related RAS genes which regulate pathways involved in cell differentiation, proliferation, adhesion, migration and apoptosis and are known proto-oncogenes. H-Ras is located within the peripheral plasma membrane and within endomembrane compartments including the ER and Golgi apparatus. In the ER H-Ras can be activated by the RasGRF family exchange factors RasGRF1 and RasGRF2, this process does not appear to happen within the Golgi (Arozarena et al. 2004). Mutations in the H-Ras gene are responsible for the rare condition known as Costello syndrome characterized by facial, cardiovascular and musculoskeletal abnormalities and mental retardation (Gripp et al. 2006).

        Mouse anti Human H-Ras antibody has been used for the detection of H-Ras in the multiple human cell line lysates by western blotting, detecting a band of ~21 kDa under reducing conditions.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "H-RAS" specificity.