H-FABP Antibody (PrecisionAbTM Antibody)

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H-FABP Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with H-FABP antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10000, STAR207P) and visualized on the ChemiDoc™ MP with 9 second exposure. Arrow points to H-FABP (molecular weight 15 kDa)

  • H-FABP Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti H-FABP
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    H-FABP antibody recognizes fatty acid-binding protein, heart, also known as fatty acid-binding protein 3, muscle, fatty acid binding protein 11, heart-type fatty acid-binding protein or mammary-derived growth inhibitor. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00102WBdatasheet pdfdatasheet pdf100 µl
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Mouse anti Human H-FABP antibody recognizes H-FABP, also known as fatty acid-binding protein 3, fatty acid binding protein 11, fatty acid-binding protein, heart-type fatty acid-binding protein, mammary-derived growth inhibitor and muscle fatty acid-binding protein.

        The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. The H-FABP gene contains four exons and its function is to arrest growth of mammary epithelial cells. The H-FABP gene is a candidate tumor suppressor gene for human breast cancer (provided by RefSeq, Jul 2008).

        Mouse anti Human H-FABP antibody detects a band of 15 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
      • Western Blotting
        Anti H-FABP detects a band of approximately 15 kDa in human heart tissue lysate
      • Product Form
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant protein corresponding to amino acids 1-133 of human H-FABP
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        negative regulation of cell proliferation
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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