GRP94 Antibody (PrecisionAbTM Antibody)

GRP94 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with GRP94 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to GRP94 (molecular weight 100 kDa)

  • GRP94 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti GRP94
  • GRP94 Antibody with Control Lysate
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    GRP94 antibody recognizes endoplasmin, also known as 94 kDa glucose-regulated protein or GRP-94 heat shock protein 90 kDa beta member 1 tumor rejection antigen 1 gp96 homolog. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00059WBdatasheet pdfdatasheet pdf100 µl
      VPA00059KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Goat anti Human GRP94 antibody recognizes GRP94 a 94 kDa glucose regulated protein (GRP94), also known as tumor rejection antigen 1 (TRA1), heat shock protein 90 kDa beta member 1 (HSP90B1) and endoplasmin. GRP94 is an endoplasmic reticulum resident molecular chaperone, which is a paralog of cytoplasmic hsp90. GRP94 assists in the folding of membrane-bound proteins including the toll-like receptors, certain subclasses of integrins and some secreted proteins such as IgG.

        Goat anti Human GRP94 detects GRP94 as a band of 100 kDa in a wide range of human cell line lysates tested. Cross reactivity with rodent cell lines is also observed.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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