GNB2L1 Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-gnb2l1-antibody-vpa00031.html?RenderPageSpecific=1
GNB2L1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with GNB2L1 antibody followed by detection with HRP conjugated Rabbit anti Goat IgG (H/L) (1/10,000, 5160-2504) and visualized on the ChemiDoc MP with 57 second exposure. Arrow points to GNB2L1 (molecular weight 35 kDa)

Enlarge
  • GNB2L1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti GNB2L1
  • GNB2L1 Antibody with Control Lysate
  • GNB2L1 Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    GNB2L1 antibody recognizes guanine nucleotide-binding protein subunit beta-2-like 1. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00031WBdatasheet pdfdatasheet pdf100 µl
      VPA00031
      VPA00031KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00031K
      VPA00031KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00031KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human GNB2L1 antibody recognizes human guanine nucleotide-binding protein subunit beta-2-like 1 (GNB2L1), also known as cell proliferation-inducing gene 21 protein, human lung cancer oncogene 7 protein, receptor for activated C kinase or RACK1. GNB2L1 is a 317 amino acid member of the WD repeat G protein beta family, containing two WD repeats. GNB2L1 acts as a substrate for Src protein-tyrosine kinase (Chang et al. 2002) and as a regulator of signaling pathways (Sato et al. 2014, Long et al. 2014).

        Goat anti Human GNB2L1 antibody recognizes human GNB2L1 as a band of ~35 kDa in multiple human cell lysates by western blotting under reducing conditions. The antibody also cross-reacts with rodent cell line lysates tested.
      • Target Species
        Human
      • Western Blotting
        Anti GNB2L1 antibody detects a band of approximately 35 kDa in HEK293 cell lysates.
        Anti GNB2L1 antibody detects a band of approximately 35 kDa in HEK293 cell lysates.
        Anti GNB2L1 antibody detects a band of approximately 35 kDa in HEK293 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Goat polyclonal antibody by purified ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody by purified ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody by purified ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody by purified ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody by purified ammonium sulfate precipitation followed by antigen affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Peptide sequence C-NTLGVCKYTVQDESH from an internal region of GNB2L1
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer.
        400μg HEK293 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Goat IgG (H/L):HRP5160-25042 mgC, E, WB
        5160-2504
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Goat IgG (H/L):HRP5160-25042 mgC, E, WB
        5160-2504
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Goat IgG (H/L):HRP5160-25042 mgC, E, WB
        5160-2504

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "GNB2L1" specificity.