GM-CSF Antibody | 3209 (30-4)

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  • Mouse anti Human GM-CSF
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3209 (30-4)
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4740-0955C, E, FN, WB*datasheet pdfdatasheet pdf0.1 mg
    4740-0955
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • 4740-0955 recognises recombinant and native human granulocyte macrophage colony stimulating factor (GM-CSF).

      Human Granulocyte colony stimulating factor (GM-CSF) is a 14.6kDa glycoprotein, which is produced by T cells, macrophages, fibroblasts and endothelial cells. GM-CSF is involved in the stimulation of proliferation and differentiation of granulocyte and macrophage progenitor cells.

      The neutralisation dose (ND50) of this batch, as determined by the proliferation of TF-1 cells, is approximately 0.3-0.5 µg/ml in the presence of 0.5 ng/ml of recombinant human GM-CSF. The ND50 may vary depending on conditions.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseWeak
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A.
    • Preservative Stabilisers
      1% trehalose
    • Immunogen
      Recombinant protein corresponding to amino acids 14 to 144 of human GM-CSF produced in E. coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      positive regulation of tyrosine phosphorylation of Stat5 protein
      granulocyte macrophage colony-stimulating factor receptor binding
      negative regulation of cytolysis
      growth factor activity
      positive regulation of interleukin-23 production
      extracellular space
      positive regulation of macrophage derived foam cell differentiation
      positive regulation of DNA replication
      immune response
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.51.0 ug/ml
      Functional Assays0.30.5 ug/ml
      Immunohistology - Frozen8.025 ug/ml
      Western Blotting(1)1.02.0 ug/ml
      (1)
      4740-0955 should be used under non-reducing conditions, reducing conditions are not recommended.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional GM-CSF Antibody Formats

    Formats Clone Applications Sizes available
    GM-CSF Antibody : Purified 3209 (30-4) C, E, FN, WB* 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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