GM-CSF Antibody (PrecisionAbTM Antibody) | 1A3

100% secure
Email this product page
Print this page
GM-CSF Antibody (PrecisionAb<sup>TM</sup> Antibody) | 1A3 gallery image 1

Western blot analysis of whole cell lysates probed with GM-CSF antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to GM-CSF (molecular weight 25 kDa)

  • GM-CSF Antibody (PrecisionAb<sup>TM</sup> Antibody) | 1A3 thumbnail image 1
  • Mouse anti GM-CSF
(Rated 0.0 out of 5 based on 0 customer reviews)
    GM-CSF antibody recognizes the granulocyte-macrophage colony-stimulating factor, also known as CSF, molgramostin or sargramostim. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00213WBdatasheet pdfdatasheet pdf100 µl
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Mouse anti Human GM-CSF antibody recognizes granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as CSF, molgramostin and sargramostim.

        Encoded by the CSF2 gene, GM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. CSF2 has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13 (provided by RefSeq, Jul 2008).

        Mouse anti Human GM-CSF antibody detects a band of 25 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
      • Western Blotting
        Anti GM-CSF detects a band of approximately 25 kDa in Mouse thymus cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Recombinant protein corresponding to amino acids 18-144 of human GM-CSF (NP_000749) produced in E. coli
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        positive regulation of DNA replication
        cytokine activity
        granulocyte macrophage colony-stimulating factor receptor binding
        immune response
        growth factor activity
        extracellular space
        positive regulation of interleukin-23 production
        negative regulation of cytolysis
        positive regulation of macrophage derived foam cell differentiation
        positive regulation of tyrosine phosphorylation of Stat5 protein
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information

                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "GM-CSF" specificity.