Western blotting analysis of human liver lysate (35μg protein in RIPA buffer) probed with Goat anti Human glutathione peroxidase 1 antibody (AHP1570) at 1.0μgml-1 . Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Goat anti Human glutathione peroxidase 1 antibody recognizes isoform 1 of human glutathione peroxidase 1 (GPX1), a ~22 kDa ubiquitously expressed selenium-dependent antioxidant enzyme and member of the glutathione peroxidase family, which catalyzes the glutathione-mediated reduction of toxic peroxides, protecting against oxidative damage and free radicals.
Genetic polymorphisms resulting in variants of GPX1 have been linked with various cancers, including lung cancer, bladder cancer, and a significantly increased risk of developing breast cancer.
Purified IgG - liquid
Antiserum to human GPX1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Peptide sequence C-REALPAPSDDATA corresponding to the internal region of glutathione peroxidase 1 (NP_000572.2).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1570 detects a band of approximately 22kDa in human liver and kidney cell lysates.