Glutamate Decarboxylase 2 Antibody (PrecisionAbTM Antibody)

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Glutamate Decarboxylase 2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with glutamate decarboxylase 2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 134 second exposure. Arrow points to glutamate decarboxylase 2 (molecular weight 60 kDa)

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  • Glutamate Decarboxylase 2 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti Glutamate Decarboxylase 2
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    Glutamate decarboxylase 2 antibody recognizes glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase or GAD-65. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00153WBdatasheet pdfdatasheet pdf100 µl
      VMA00153
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human glutamate decarboxylase 2 antibody recognizes glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, GAD-65, or glutamate decarboxylase 65 kDa isoform.

        Encoded by the GAD2 gene, glutamate decarboxylase 2 is one of several forms of glutamic acid decarboxylase. Glutamate decarboxylase 2 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein (provided by RefSeq, Oct 2008).

        Mouse anti Human glutamate decarboxylase 2 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti glutamate Decarboxylase 2 detects a band of approximately 60 kDa in Mouse Brain cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Keyhole limpet haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human glutamate decarboxylase 2
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        neurotransmitter secretion
        pyridoxal phosphate binding
        glutamate decarboxylation to succinate
        cell junction
        clathrin sculpted gamma-aminobutyric acid transport vesicle membrane
        presynaptic membrane
        cytosol
        glutamate decarboxylase activity
        protein binding
        neurotransmitter biosynthetic process
        Golgi membrane
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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