Glucose Transporter 4 Antibody

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Staining of paraffin embedded human heart, demonstrating labelling of cardiac myocytes

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Published customer image:
Rabbit anti Glucose Transporter 4 antibody (4670-1704) used to evaluate GLUT4 expression in rat soleus muscle by western blotting
Image caption:
(A) Basal and insulin-stimulated glucose uptakes in rat soleus muscle in contralateral non-immobilized and immobilized limbs at the end of 6-h hindlimb immobilization. Muscles were dissected out at the end of 6-h unilateral hindlimb immobilization. All muscles were incubated in glucose-free medium in the absence or presence (50 or 10,000 μU/mL) of insulin for 20 min, followed by the measurement of 2-deoxyglucose uptake. Values are means ± SE (n = 5–6). *P <0.05 versus contralateral non-immobilized limbs with same insulin concentration. † P <0.05 versus 0 μU/mL insulin. (B) Total GLUT4 protein concentration at the end of 6-h hindlimb immobilization. Muscles were dissected out and frozen at the end of 6-h unilateral hindlimb immobilization. Muscle lysates were separated with SDS-PAGE, and blots were analyzed for GLUT4 protein content. Values are means ± SE (n = 8).

From: Kawamoto E, Koshinaka K, Yoshimura T, Masuda H,
Kawanaka K. Immobilization rapidly induces muscle insulin resistance together with the activation of MAPKs (JNK and p38) and impairment of AS160 phosphorylation.
Physiol Rep. 2016 Aug;4(15). pii: e12876..

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  • Rabbit anti Glucose Transporter 4
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4670-1704C, Pdatasheet pdfdatasheet pdf0.1 ml
    4670-1704
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Glucose Transporter 4 antibody recognizes human Solute carrier family 2, facilitated glucose transporter member 4, also known as glucose transporter 4 or GLUT4. GLUT4 is a 509 amino acid ~50 kDa multi-pass transmembrane protein located primarily in the perinuclear region and is translocated to the plasma membrane where it is rapidly reinternalized. Rabbit anti Glucose Transporter 4 antibody does not react with HEP G2 type glucose transporter in human erythrocytes, rat brain or other tissues that do not exhibit sensitivity to insulin.

      GLUT4 is the insulin-regulated glucose transporter found in adipose tissue and striated muscle, responsible for glucose disposal. The stimulation of glucose uptake by insulin requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      Pigno
      Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      A 12 amino acid peptide corresponding to the carboxyl terminus of the insulin regulatable glucose transporter conjugated to keyhole limpet hemocyanin.
    • Purity
    • Approx. Protein Concentrations
      5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein binding
      external side of plasma membrane
      D-glucose transmembrane transporter activity
      integral to plasma membrane
      carbohydrate metabolic process
      perinuclear region of cytoplasm
      glucose import
      glucose homeostasis
      vesicle membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin1020 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Glucose Transporter 4 Antibody Formats

    Formats Applications Sizes available
    Glucose Transporter 4 Antibody : Purified C, P 0.1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting

              References

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