Glucagon Antibody

//www.bio-rad-antibodies.com/human-glucagon-antibody-4660-1140.html?RenderPageSpecific=1
Glucagon Antibody gallery image 1

Published customer image:
Goat anti Human glucagon antibody (4660-1140) used for the demonstration of glucagon expressing cells in murine embryonic tissue sections by immunofluorescence.
Image caption:
Spatiotemporal expression of Sel1l during development of the mouse pancreas. Pancreatic sections from timed Sel1l+/βgeo embryos were co-immunostained with antibodies against βGal (red) and various pancreatic markers (green) as indicated on the left side of each panel. (B and H) Magnified views of the boxed area in A and G, respectively. (A-D) βGal expression at E12.5. βGal expression begins in a small number of cells within the core pancreatic epithelium at E12.5 (A). At this stage, there is no co-expression of βGal and SOX9 (B); however, βGal is co-expressed with either glucagon (C) or insulin (D). (E-F) βGal expression at E14.5. βGal is differentially expressed in the PDX1+ cells (E). While high βGal signal was detected in the epithelial branches (arrows), no or very low Gal signal was detected in the core "duct-like" epithelium (arrowhead). These core epithelial cells correspond to the subset of PDX+ cells that express SOX9 (F). (G-J) βGal expression at E15.5. βGal expression increases markedly in the core pancreatic epithelium (G and H, asterisks). βGal co-localizes with either glucagon (I, arrows) or insulin (J, arrows). (K-L) βGal expression at postnatal day 1. βGal is expressed in both islet and acinar tissues (K, asterisk and arrow, respectively). βGal co-localizes with PDX1 in the islet tissue (L). Scale bars: (A, G, I and J) 50 μm; (B-F, K-L) 100 μm.

From: Li S, Francisco AB, Munroe RJ, Schimenti JC, Long Q. SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.
BMC Dev Biol. 2010 Feb 19;10:19.

Enlarge
Glucagon Antibody gallery image 2

Published customer image:
Goat anti Human glucagon antibody (4660-1140) used for the demonstration of glucagon expressing cells in murine embryonic tissue sections by immunofluorescence.
Image caption:
Inhibited endocrine and exocrine cell differentiation in Sel1lβgeo/βgeo embryos. (A-F) Immunohistological analysis of the developing pancreas of Sel1l+/+ and Sel1lβgeo/βgeo embryos at E11.5 (A-B) and E15.5 (C-F); the genotypes of the pancreatic sections are indicated as "+/+" and "-/-", respectively. The following antibodies were used: Pdx1 (A-D, green), glucagon (A-D, red), insulin (E-F, red) and amylase (E-F, green). (A-B) At E11.5, Glu+ cells were detected in the pancreatic epithelium of wild-type and Sel1lβgeo/βgeo embryos in equal numbers. (C-D) At E15.5, the number of glucagon+ cells was reduced in Sel1lβgeo/βgeo embryos as compared to wild-type control. (E-F) No amylase+ cells were detected and the number of insulin+ cells was significantly reduced in Sel1lβgeo/βgeo embryos. (G-I) Statistical analyses of the numbers of glucagon+ and insulin+ cells in wild-type and Sel1lβgeo/βgeo embryos. Data were from three wild-type and three Sel1lβgeo/βgeo embryos. At E11.5, no significant difference in the number of glucagon+ cells in wild-type and Sel1lβgeo/βgeo embryos was detected (G). The number of glucagon+ and insulin+ cells was markedly lower in Sel1lβgeo/βgeo embryos than in wild-type control embryos (H and I, respectively). Scale bar: 100 μm.

From: Li S, Francisco AB, Munroe RJ, Schimenti JC, Long Q. SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.
BMC Dev Biol. 2010 Feb 19;10:19.

Enlarge
  • Glucagon Antibody thumbnail image 1
  • Glucagon Antibody thumbnail image 2
  • Goat anti Human Glucagon (N-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4660-1140Edatasheet pdfdatasheet pdf1 ml
    4660-1140
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Human glucagon antibody recognizes the pancreatic polypeptide hormone glucagon, whose actions are antagonistic to insulin. Glucagon is produced by A cells within the Islets of Langerhans in the pancreas.

      Tumors arising from glucagon producing cells are termed glucagonomas, and this antibody may be useful in identifying such tumours.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Porcine glucagon, amino acids 1-12, conjugated with bovine thyroglobulin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      regulation of insulin secretion
      extracellular region
      cellular response to glucagon stimulus
      soluble fraction
      energy reserve metabolic process
      feeding behavior
      G-protein coupled receptor protein signaling pathway
      hormone activity
      cell proliferation
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Glucagon Antibody Formats

    Formats Applications Sizes available
    Glucagon Antibody : Purified E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...