GFAP Antibody

GFAP Antibody gallery image 1

Western blotting analysis of human cerebellum (A), Mouse brain (B) and Rat brain (C) lysates (35µg protein in RIPA buffer) probed with Goat anti Human GFAP antibody (AHP1468) at 0.01, 0.3 and 0.01μgml-1 respectively. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

GFAP Antibody gallery image 2

Immunohistochemical staining of formalin fixed cryosection of human hypothalamus following antigen retrieval with citrate buffer pH 6.0 at 80°C for 30 min. Sections stained with Goat anti Human GFAP (AHP1468) at 0.01 μgml-1 followed by secondary anti Goat-HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

GFAP Antibody gallery image 3

Immunohistochemical staining of paraffin embedded human cerebellum following heat mediated antigen retrieval in citrate buffer pH 6.0 with Goat anti Human GFAP antibody at 2μgml-1 followed by secondary anti Goat-HRP and DAB chromogen visualization

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  • Goat anti Human GFAP (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1468E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human GFAP antibody recognizes an epitope within the C-terminal (CT) region of human GFAP (glial fibrillary acidic protein), a class III intermediate filament (IF) protein specifically expressed by glial cells or cells of glial origin e.g astrocytes, ependymal cells and Schwann cells.

      GFAP plays a role in several cellular functions within the central nervous system (CNS), including cell structure and stability, communication, motility and mitosis, and is rapidly synthesized during astrogliosis, following trauma/injury. Mutations in the GFAP gene are responsible for the rare autosomal dominant disorder known as Alexander disease, resulting in the destruction of brain white matter and the formation of fibrous, eosinophilic deposits known as Rosenthal fibers. Characteristics of this disease are associated with transgenes and other mutation types in mouse.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      RatExpected from Sequence
      DogExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human GFAP (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Peptide sequence C-DGEVIKESKQEHKD from the C-terminal region of GFAP (NP_002046.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.03.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1468 detects a band of approximately 48kDa in mouse brain cell lysates.
    • Instructions For Use

    Additional GFAP Antibody Formats

    Formats Applications Sizes available
    GFAP Antibody : Purified E, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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