Glial cell-derived neurotrophic factor (GDNF), is 30kDa neurotrophic factor and member of the cysteine knot superfamily of growth factors.
GDNF binds GDNF alpha receptor which leads to the activation of Ret protein tyrosine kinase receptor, promoting dopamine uptake and the survival and morphological differentiation of midbrain neurons. GDNF has been shown to improve conditions such as bradykinesia, rigidity and postural instability in Parkinson’s disease mouse models. Defects in the GDNF gene may cause Hirschsprung disease and congenital central hypoventilation syndrome.
Purified recombinant protein - lyophilised
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant human GDNF, expressed in E. coli
Determined by the dose-dependent dopamine uptake by rat mesencephalic cultures was found to be in the range of 5-10 ng/ml.
>98% by SDS PAGE and HPLC analysis.
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution.
Reagents In The Kit
Preparing The Antibody
Citrate buffered saline
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
PHP165 may be used as a standard in ELISA applications with AHP365G
Histology Positive Control Tissue
PHP165 may be used as a positive control under reducing or non-reducing Western blotting conditions with AHP365G