GCH1 Antibody | 4A12

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GCH1 Antibody | 4A12 gallery image 1

Staining of paraffin embedded human lymph node with Mouse anti Human GCH1:Azide Free (MCA3138Z)

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GCH1 Antibody | 4A12 gallery image 2

Western blot analysis of GCH1 expression in IMR-32 using Mouse anti Human GCH1:Azide Free (MCA3138Z)

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  • Mouse anti Human GCH1:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    4A12
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3138ZP *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3138Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human GCH1 monoclonal antibody, clone 4A12 recognizes guanosine triphosphate cyclohydrolase 1, also known as GTP cyclohydrolase 1 or GTP-CH-I, encoded by the GCH1 gene. GTP cyclohydrolase 1 has multiple potential isoforms but only isoform 1 gives rise to an active form of the enzyme (Gütlich et al. 1994). GTP cyclohydrolase 1 has a predicted molecular weight of ~28kDa, possesses three zinc binding sites and has two potential phosphoserine residues (Widder et al. 2007) .

      GTP-CH 1 is the initial rate limiting factor involved in the biosynthesis of tetrahydrobiopterin (BH4), itself an essential co-factor in the generation of active forms of nitric oxide synthase and in catecholamine and 5-hydroxytryptamine (serotonin) biosynthesis (Thöny et al. 200).

      Deficiency of GTP-CH1 is implicated in malignant hyperphenylalaninemia due to tetrahydrobiopterin deficiency (Ichinose et al. 1995), whilst abnormalities of the GCH1 gene have also been documented for their involvement in locomotor pathologies including dystonia 5, a condition affecting gait and posture with marked fluctuating diurnal presentation (Hu et al. 2010).

      Mouse anti human GCH1 has been used effectively for the identification of GTP cyclohydrolase 1 expression in cells using Western blotting (Ryan et al. 2013).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml
      Immunohistology - Paraffin(1)0.110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional GCH1 Antibody Formats

    Formats Clone Applications Sizes available
    GCH1 Antibody : Preservative Free 4A12 P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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