GATA1 (pSer310) Antibody

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GATA1 (pSer310) Antibody gallery image 1

Western blot analysis of extracts from HT-29 cells untreated or treated with Anisomycin using Rabbit anti GATA1 (pSer310) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Anisomycin (5ug/ml) for 30 minutes

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  • Rabbit anti GATA1 (pSer310)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2624WBdatasheet pdfdatasheet pdf50 µl
    AHP2624
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti GATA1 (pSer310) antibody recognizes GATA1 when phosphorylated at serine 310. GATA1 is a member of the GATA transcription factor family and plays a significant role in the maturation of blood cells such as red blood cells, mast cells and eosinophils. Phosphorylation of GATA1 at serine 310 occurs via activation of AKT. Phosphorylation of GATA1 at serine 310 plays a role in normal and pathogenic erythopoietis.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to GATA1 (pSer310) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
      50% Glycerol
    • Immunogen
      Phospho specific-peptide corresponding to residues surrounding serine 310 of human GATA1
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      nucleolus
      multicellular organismal development
      regulation of transcription from RNA polymerase II promoter
      zinc ion binding
      blood coagulation
      nucleoplasm
      sequence-specific DNA binding transcription factor activity
      nuclear membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional GATA1 (pSer310) Antibody Formats

    Formats Applications Sizes available
    GATA1 (pSer310) Antibody : Purified WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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