GATA1 Antibody

//www.bio-rad-antibodies.com/human-gata1-antibody-ahp2401.html
GATA1 Antibody gallery image 1

Immunofluorescent analysis of GATA1 (green) in A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GATA1 polyclonal antibody in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated cells were counterstained with phalloidin (F-actin, red) and Hoechst 33342 (Nucleus, blue). Images were taken at a magnification of 60x

Enlarge
GATA1 Antibody gallery image 2

Immunofluorescent analysis of GATA1 (green) in U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GATA1 polyclonal antibody in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated cells were counterstained with phalloidin (F-actin, red) and Hoechst 33342 (Nucleus, blue). Images were taken at a magnification of 60x

Enlarge
GATA1 Antibody gallery image 3

Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human breast tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting

Enlarge
GATA1 Antibody gallery image 4

Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human brain tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting

Enlarge
GATA1 Antibody gallery image 5

Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human tonsil tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting

Enlarge
GATA1 Antibody gallery image 6

Western blot analysis of GATA1 was performed by loading 25 ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with rabbit polyclonal antibody recognizing GATA1 at a dilution of 1/500 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1% Tween 20 and probed with goat anti-rabbit-HRP secondary antibody for at least one hour. Membranes were washed and detection was carried out using chemiluminescence

Enlarge
  • GATA1 Antibody thumbnail image 1
  • GATA1 Antibody thumbnail image 2
  • GATA1 Antibody thumbnail image 3
  • GATA1 Antibody thumbnail image 4
  • GATA1 Antibody thumbnail image 5
  • GATA1 Antibody thumbnail image 6
  • Rabbit anti GATA1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2401IC, IF, P *, WBdatasheet pdfdatasheet pdf50 µg
    AHP2401
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti GATA1 antibody recognizes GATA1, also known as GATA binding factor 1. The protein is a member of the GATA family of transcription factors, which has received its name from binding DNA containing the GATA consensus motif 5'-[AT]GATA[AG]-3'  (uniprot P15976).

      GATA1 acts as an erythroid transcription factor by regulating erythroid-specific genes. In addition to that, GATA1 has also been implied to play a role in regulating differentiation of other hematopoietic lineages (including mast cells and megakaryocytes) and endothelial cells (Orkin 1992).

      GATA1 alterations have been implicated in a variety of diseases, including acute megakaryoblastic leukemia (AMKL) associated with Trisomy 21 and thrombocytopenia (Ciovacco et al. 2008).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to GATA1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
    • Preservative Stabilisers
      0.05% Sodium Azide (NaN3)
      1 mg/ml Bovine Serum Albumin
      30% Glycerol
    • Immunogen
      N-terminal truncated human recombinant protein
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      nucleolus
      blood coagulation
      regulation of transcription from RNA polymerase II promoter
      sequence-specific DNA binding transcription factor activity
      multicellular organismal development
      zinc ion binding
      nucleoplasm
      nuclear membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunocytochemistry1/501/500
      Immunofluorescence1/501/500
      Western Blotting1/500
      Immunohistology - Paraffin(1)1/501/500
      (1)
      This antibody requires heat-mediated antigen retrieval prior to staining. Bio-Rad recommend citrate buffer pH 6.0 for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti GATA1 antibody recognizes a band of approximately 43 kDa in NCCT cell lysate, NTERRA cell lysate and K562 cell lysate.
    • Instructions For Use

    Additional GATA1 Antibody Formats

    Formats Applications Sizes available
    GATA1 Antibody : Purified IC, IF, P *, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...