Galectin-1

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  • Recombinant Human Galectin-1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP187E, FN, WBdatasheet pdfdatasheet pdf50 µg
    PHP187
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human galectin-1 (Gal-1) is a member of the galectin family of beta-galactoside-binding proteins, which exhibits both intracellular and extracellular functions, and is differentially expressed by a wide range of normal and neoplastic tissues.

      Galectin-1 has been implicated in several major cellular functions including proliferation, motility, haematopoietic cell differentiation, apoptosis promotion and immunoregulatory effects. The expression of Gal-1 by neuronal and glial cells correlates with neuroregeneration following injury, and studies have shown the importance of Gal-1 to primary sensory neurons and synaptic connections in the spinal cord, during embryonic development.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the additiona of 0.1% bovine serum albumin (BSA) is recommended.
    • Preparation
      Purified recombinant galectin-1 expressed in E.coli
    • Preservative Stabilisers
      None present
    • Activity
      Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 µg/ml with a peak response obtained at 250 µg/ml.
    • Purity
      >98% by SDS PAGE/HPLC analysis
    • Approx. Protein Concentrations
      Total protein concentration 0.1 mg/ml after reconstitution
    • Molecular Weight
      14.5kD (134 amino acid residues)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      < 1 EU/ug
    • Test Principle
    • Buffer Solution
      10mM Sodium phosphate pH7.5
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      cytoplasm
      regulation of apoptosis
      apoptosis
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      proteinaceous extracellular matrix
      galactoside binding
      extracellular space
      signal transducer activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant human galectin-1 may be used as the standard for ELISA applications with either our purified human galectin-1 antibody (AHP1480) or our biotinylated human galectin-1 antibody (AHP1480B)
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant human galectin-1 may be used as the positive control for Western Blotting applications with either our purified human galectin-1 antibody (AHP1480) or our biotinylated human galectin-1 antibody (AHP1480B).
    • Instructions For Use

    Additional Galectin-1 Formats

    Formats Applications Sizes available
    Galectin-1 : Rec. Protein E, FN, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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