Western blotting analysis of HEPG2 cell lysate (35µg protein in RIPA buffer) probed with Goat anti Human GADD34 antibody (AHP683) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Immunohistochemical staining of paraffin embedded human liver probed with Goat anti Human GADD34 antibody (AHP683) following heat mediated antigen retrieval with citrate buffer pH 6.0, Detection with anti Goat secondary conjugated to HRP with DAB chromogen development
Goat anti Human GADD34 antibody recognizes the protein GADD34 (growth arrest and DNA-damage-inducible 34), also known as PPP1R15A. GADD34 is a member of a group of genes whose transcript levels are increased in response to treatment with DNA damaging agents or conditions of stress (Amundson et al. 1998). Induction of GADD34 by ionising radiation has been reported in some cell lines, regardless of p53 status (Hollander et al. 1997). Reports suggest that GADD34 may be involved in a signal transduction pathway controlling cell cycle arrest and/or apoptosis following DNA damage (Hollander et al. 2003).
Goat anti human GADD34 antibody has been used successfully for following GADD34 protein turnover in multiple cell lines and primary cells by Western blotting (Zhou et al. 2013)
Purified IgG - liquid
Antisera to GADD34 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Bovine Serum Albumin
Peptide sequence AAALDLSGRRG corresponding to the C-terminus of GADD34 (NP_055145).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
Histology Positive Control Tissue
AHP683 detects a band of approximately 80-90kDa in U937 cell lysates. An overnight incubation time of 12 hours is recommended for this antibody.