GADD34 Antibody

GADD34 Antibody gallery image 1

Western blot analysis of HeLa whole cell lysate probed with Goat anti Human GADD34 (AHP683) followed by Donkey anti Sheep/Goat IgG:HRP (STAR88P)

GADD34 Antibody gallery image 2

Western blotting analysis of HEPG2 cell lysate (35μg protein in RIPA buffer) probed with Goat anti Human GADD34 antibody (AHP683) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

GADD34 Antibody gallery image 3

Immunohistochemical staining of paraffin embedded human liver probed with Goat anti Human GADD34 antibody (AHP683) following heat mediated antigen retrieval with citrate buffer pH 6.0, Detection with anti Goat secondary conjugated to HRP with DAB chromogen development

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  • Goat anti Human GADD34
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP683WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human GADD34 antibody recognizes the protein GADD34 (growth arrest and DNA-damage-inducible 34), also known as PPP1R15A. GADD34 is a member of a group of genes whose transcript levels are increased in response to treatment with DNA damaging agents or conditions of stress (Amundson et al. 1998). Induction of GADD34 by ionising radiation has been reported in some cell lines, regardless of p53 status (Hollander et al. 1997). Reports suggest that GADD34 may be involved in a signal transduction pathway controlling cell cycle arrest and/or apoptosis following DNA damage (Hollander et al. 2003).

      Goat anti human GADD34 antibody has been used successfully for following GADD34 protein turnover in multiple cell lines and primary cells by Western blotting (Zhou et al. 2013)
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to GADD34 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide sequence AAALDLSGRRG corresponding to the C-terminus of GADD34 (NP_055145).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      endoplasmic reticulum
      response to DNA damage stimulus
      protein binding
      regulation of translation
      cell cycle arrest
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.5ug/ml2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP683 detects a band of approximately 80-90kDa in U937 cell lysates. An overnight incubation time of 12 hours is recommended for this antibody.
    • Instructions For Use

    Additional GADD34 Antibody Formats

    Formats Applications Sizes available
    GADD34 Antibody : Purified WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting


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