GABPA Antibody | 5C8

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GABPA Antibody | 5C8 gallery image 1

Western Blot analysis of GABPA expression in Hela NE using Mouse anti Human GABPA (MCA4907Z)

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GABPA Antibody | 5C8 gallery image 2

Staining of paraffin-embedded human stomach with Mouse anti Human GABPA (MCA4907Z)

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GABPA Antibody | 5C8 gallery image 3

Immunofluorescence showing GABPAin HeLa cells using Mouse anti Human GABPA (MCA4907Z)

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  • Mouse anti Human GABPA:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5C8
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4907ZIF, P, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4907Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human GABPA antibody, clone 5C8 recognizes human GA-binding protein alpha chain, also known as GAPBA, Nuclear respiratory factor 2 subunit alpha or Transcription factor E4TF1-60. GAPBA is a 454 amino acid transcription factor. GAPBA is required for expression of the Adenovirus E4 gene and is an important regulator during embryogenesis (Ristevski et al. 2004).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1-101 of human GABPA
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      positive regulation of gene-specific transcription from RNA polymerase II promoter
      nucleus
      RNA polymerase II transcription factor activity
      protein heterodimerization activity
      promoter binding
      transcription from RNA polymerase II promoter
      transcription coactivator activity
      transcription activator activity
      sequence-specific DNA binding transcription factor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Immunohistology - Paraffin0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional GABPA Antibody Formats

    Formats Clone Applications Sizes available
    GABPA Antibody : Preservative Free 5C8 IF, P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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