G3BP1 Antibody (PrecisionAbTM Antibody)

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G3BP1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with G3BP1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 6 second exposure. Arrow points to G3BP1 (molecular weight 68 kDa)

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  • Rabbit anti G3BP1
  • G3BP1 Antibody with Control Lysate
  • G3BP1 Antibody with Control Lysate
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    Rabbit anti-human G3BP1 antibody recognizes Ras GTPase-activating protein-binding protein 1, also known as ATP-dependent DNA helicase VIII, GAP SH3 domain-binding protein 1 or GAP binding protein. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00492WBdatasheet pdfdatasheet pdf100 µl
      VPA00492
      VPA00492KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00492K
      VPA00492KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00492KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human G3BP1 antibody recognizes G3BP1 also known as, Ras GTPase-activating protein-binding protein 1, ATP-dependent DNA helicase VIII, G3BP-1, GAP SH3 domain-binding protein 1, GAP binding protein, Ras-GTPase-activating protein SH3-domain-binding protein or RasGAP-associated endoribonuclease G3BP.

        G3BP1 gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of G3BP1 have been described, but the full-length nature of some of these variants has not been determined (provided by RefSeq, Jul 2008).

        Rabbit anti Human G3BP1 antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti G3BP1 detects a band of approximately 68 kDa in Jurkat cell lysates
        Anti G3BP1 detects a band of approximately 68 kDa in Jurkat cell lysates
        Anti G3BP1 detects a band of approximately 68 kDa in Jurkat cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        2% Sucrose
        0.09% Sodium Azide (NaN3)
        2% Sucrose
        0.09% Sodium Azide (NaN3)
        2% Sucrose
      • Immunogen
        Synthetic peptide directed towards the N terminal region of human G3BP1
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer.
        400μg Jurkat lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        Ras protein signal transduction
        nucleus
        cytosol
        DNA binding
        ATP-dependent DNA helicase activity
        ATP-dependent RNA helicase activity
        protein binding
        transport
        endonuclease activity
        ATP binding
        plasma membrane
        RNA binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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