FYN Antibody (PrecisionAbTM Antibody)

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FYN Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with FYN antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 33 second exposure. Arrow points to FYN (molecular weight 60 kDa)

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  • FYN Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • FYN Antibody with Control Lysate
  • Goat anti FYN
  • FYN Antibody with Control Lysate
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    Fyn antibody recognizes tyrosine-protein kinase Fyn, also known as proto-oncogene Syn, proto-oncogene c-Fyn, Src-like kinase, SLK or p59-Fyn. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00019WBdatasheet pdfdatasheet pdf100 µl
      VPA00019
      VPA00019KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00019K
      VPA00019KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00019KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human FYN antibody recognizes an epitope within the N-terminal (NT) region of proto-oncogene Tyrosine-protein kinase Fyn encoded by the FYN gene, also known as proto-onocgene Syb or c-Fyn. Human c-Fyn is a 537 amino acid, ~60 kDa, cell membrane non-receptor protein tyrosine kinase and member of the Tyr protein kinase family, SRC subfamily. Fyn contains single protein kinase, SH2 and SH3 domains and shares strong similarities with mouse FYN, v-yes and c-src.

        c-Fyn plays roles in many biological processes including cell growth (Yasunaga et al. 1996), adhesion (Chapman et al. 2012), re-modelling (Wang et al. 2012), motility (Yadav et al. 2011) and the immune response (Oykhman et al. 2013).

        The immunogen sequence used for the generation of this Goat anti c-Fyn polyclonal antibody is well conserved across a very wide range of mammalian species and the polyclonal anti c-Fyn antibody is expected to have a wide species pattern of reactivity.

        Goat anti Human FYN antibody recognizes human FYN as a single band of ~60 kDa in multiple human cell line lysates by western blotting under reducing conditions. It also cross-reacts with rodent cell line lysates tested.
      • Target Species
        Human
      • Western Blotting
        Anti FYN antibody detects a band of approximately 60 kDa in HepG2 cell lysates.
        Anti FYN antibody detects a band of approximately 60 kDa in HepG2 cell lysates.
        Anti FYN antibody detects a band of approximately 60 kDa in HepG2 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
        Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Peptide sequence C-VQCKDKEATKLTE from the N-terminal region of FYN
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HepG2 lysate lyophilized in RIPA buffer.
        400μg HepG2 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        T cell receptor signaling pathway
        ATP binding
        glycoprotein binding
        calcium ion transport
        regulation of defense response to virus by virus
        cytosol
        endosome
        T cell costimulation
        protein phosphorylation
        learning
        non-membrane spanning protein tyrosine kinase activity
        intracellular protein kinase cascade
        plasma membrane
        platelet activation
        axon guidance
        metal ion binding
        feeding behavior
        interspecies interaction between organisms
        identical protein binding
        leukocyte migration
        viral reproduction
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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