Furin Antibody

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Paraffin embedded human liver stained with Mouse anti Human furin aa553-565 (AHP2265)

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  • Goat anti Human Furin (aa553-565)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2265E, P *datasheet pdfdatasheet pdf0.1 mg
    AHP2265
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human furin antibody recognizes an epitope corresponding to aa553-565 of human Furin, otherwise known as PACE (Paired basic Amino acid Cleaving Enzyme). Furin is a calcium-dependent serine endoprotease and proprotein convertase, which plays a crucial role in the processing of latent precursor proteins into their biologically active forms, such as during the Notch signaling pathway.

      The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T cell development, and both tumor progression and suppression.

      Furin is a critical enzyme during the S1 cleavage of pre-Notch proteins within the golgi apparatus, to convert the nascent form into the mature heterodimeric Notch receptor and the non-covalently associated intracellular domain. These are then transported to the cell surface, in preparation for ligand-receptor binding and further S2 and S3 cleavage, resulting in the release and translocation of the intracellular domain to the nucleus.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/8000
      Western Blotting
      Immunohistology - Paraffin(1)2.04.0ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human liver
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Furin Antibody Formats

    Formats Applications Sizes available
    Furin Antibody : Purified E, P * 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human liver

              Further Reading

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