FSCN1 Antibody (PrecisionAbTM Antibody) | CPTC29

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FSCN1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | CPTC29 gallery image 1

Western blot analysis of whole cell lysates probed with FSCN1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to FSCN1 (molecular weight 55 kDa)

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  • FSCN1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | CPTC29 thumbnail image 1
  • Mouse anti FSCN1
  • FSCN1 Antibody with Control Lysate
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  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG2a
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00557WBdatasheet pdfdatasheet pdf100 µl
    VMA00557
    VMA00557KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00557KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Mouse anti Human FSCN1 antibody recognizes Fascin, also known as 55 kDa actin-bundling protein, fascin homolog 1, actin-bundling protein, singed-like (fascin homolog, sea urchin).

      The FSCN1 gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of FSCN1 is known to be regulated by several microRNAs, and overexpression of FSCN1 may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of FSCN1 is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of FSCN1 is located on the long arm of chromosome 15 (provided by RefSeq, Sep 2011).

      Mouse anti Human FSCN1 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Anti FSCN1 detects a band of approximately 55 kDa in K562 cell lysates
      Anti FSCN1 detects a band of approximately 55 kDa in K562 cell lysates
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant protein of human FSCN1
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg K562 lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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