Fractalkine Antibody

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  • Rabbit anti Human Fractalkine:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP453BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP453B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human Fractalkine antibody recognizes human fractalkine, otherwise known as CX3CL1, a 397 amino acid CX3C chemokine which signals through the CX3CR1 receptor and is expressed by various tissues including the brain, testis, lung, heart, kidney, colon and skeletal muscle.

      Adhesion of fractalkine to the CX3CR1 receptor triggers the chemotaxis and adhesion of leucocytes, including T cells, monocytes and NK cells and studies have suggested that fractalkine is involved in the regulation of brain microglia during neuroinflammatory processes (Sunnemark et al. 2005). CX3CL1 may also play a role in dendritic cellmediated host defense against invading pathogens such as Legionella pneumophila (Kikuchi et al. 2005).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin.
    • Preparation
    • Antiserum Preparation
      Antisera to human fractalkine were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant human fractalkine
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 50ug/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage, the addition of 0.09% sodium azide is recommended.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      plasma membrane
      integral to membrane
      positive regulation of calcium-independent cell-cell adhesion
      cell surface
      cell adhesion
      positive regulation of inflammatory response
      chemokine activity
      leukocyte adhesive activation
      cytokine-mediated signaling pathway
      extracellular space
      immune response
      defense response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.150.30ug/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Fractalkine Antibody Formats

    Formats Applications Sizes available
    Fractalkine Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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