Rabbit anti Human Fractalkine antibody recognizes human fractalkine, otherwise known as CX3CL1, a 397 amino acid CX3C chemokine which signals through the CX3CR1 receptor and is expressed by various tissues including the brain, testis, lung, heart, kidney, colon and skeletal muscle.
Adhesion of fractalkine to the CX3CR1 receptor triggers the chemotaxis and adhesion of leucocytes, including T cells, monocytes and NK cells and studies have suggested that fractalkine is involved in the regulation of brain microglia during neuroinflammatory processes (Sunnemark et al. 2005). CX3CL1 may also play a role in dendritic cellmediated host defense against invading pathogens such as Legionella pneumophila (Kikuchi et al. 2005).
Purified IgG conjugated to Biotin - lyophilised
Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin.
Antisera to human fractalkine were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Recombinant human fractalkine
Approx. Protein Concentrations
IgG concentration 50ug/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage, the addition of 0.09% sodium azide is recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
1. Sunnemark, D. et al. (2005) CX3CL1 (fractalkine) and CX3CR1 expression in myelin oligodendrocyte glycoprotein-induced experimental immune encephalomyelitis: kinetics and cellular origin. J. Neuroinflamm. 2: 17.
2. Kikuchi, T. et al. (2005) Involvement of Fractalkine/CX3CL1 expression by dendritic cells in the enhancement of host immunity against Legionella pneumophila.Infect. Immun. 73: 5350-5357.