Western blotting analysis of human cerebellum lysate (35μg protein in RIPA buffer) probed with Goat anti Human FOCP2 antibody (AHP2020) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Goat anti Human FOXP2 antibody recognizes Forkhead box protein P2, also known as FOXP2, CAG repeat protein 44 or Trinucleotide repeat-containing gene 10 protein. FOXP2 is a 715 amino acid ~80 kDa nuclear transcriptional repressor with a role in brain and lung development.
Mutations in the FOXP2 gene has been identified Speech-language disorder 1 (SPCH1), a disorder characterized by severe orofacial dyspraxia with speech impairment (Lai et al. 2001).
Multiple potential isoforms of FOXP2 generated by alternative splicing have been described. Goat anti Human FOXP2 antibody (AHP2020) is expected to recognize the canonical isoform 1 and also isoforms 3 and 9. However the antibody is not expected other isoforms which lack the c-terminal region (UniProt : 015409).
Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antiserum to human FOXP2 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Peptide with sequence C-REIEEEPLSEDLE from the C Terminus of FOXP2.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP2020 detects a band of approximately 80kDa in human cerebellum cell lysates.