FOXA2 Antibody (PrecisionAbTM Antibody) | OTI3C10

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FOXA2 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI3C10 gallery image 1

Western blot analysis of whole cell lysates probed with FOXA2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 46 second exposure. Arrow points to FOXA2 (molecular weight 50 kDa)

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  • FOXA2 Antibody with Control Lysate
  • FOXA2 Antibody with Control Lysate
  • Mouse anti FOXA2
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    FOXA2 antibody recognizes hepatocyte nuclear factor 3-beta, also known as HNF-3-beta, HNF-3B, TCF-3B, forkhead box protein A2, hepatic nuclear factor-3-beta or transcription factor 3B. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00137WBdatasheet pdfdatasheet pdf100 µl
      VMA00137
      VMA00137KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00137K
      VMA00137KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00137KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human FOXA2 antibody recognizes FOXA2, also known as HNF-3-beta, HNF-3B, TCF-3B, forkhead box protein A2, hepatic nuclear factor-3-beta, hepatocyte nuclear factor 3, beta, hepatocyte nuclear factor 3-beta and transcription factor 3B.

        The FOXA2 gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA2 has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for FOXA2 (provided by RefSeq, Oct 2008).

        Mouse anti Human FOXA2 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti FOXA2 detects a band of approximately 50 kDa in HepG2 cell lysates.
        Anti FOXA2 detects a band of approximately 50 kDa in HepG2 cell lysates.
        Anti FOXA2 detects a band of approximately 50 kDa in HepG2 cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Mouse monoclonal antibody purified by affinity chromatography from ascites.
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HepG2 lysate lyophilized in RIPA buffer.
        400μg HepG2 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        endocrine pancreas development
        cytoplasm
        transcription factor complex
        transcription factor binding
        dorsal/ventral neural tube patterning
        endoderm development
        in utero embryonic development
        positive regulation of gene-specific transcription from RNA polymerase II promoter
        regulation of transcription factor activity
        ectoderm formation
        lung epithelial cell differentiation
        protein domain specific binding
        oligodendrocyte cell fate commitment
        epithelial tube branching involved in lung morphogenesis
        specific RNA polymerase II transcription factor activity
        sequence-specific enhancer binding RNA polymerase II transcription factor activity
        chromatin modification
        specific transcriptional repressor activity
        transcription activator activity
        positive regulation of neuron differentiation
        negative regulation of gene-specific transcription from RNA polymerase II promoter
        anterior/posterior pattern formation
        negative regulation of neuron differentiation
        neuron fate specification
        regulation of blood coagulation
        central nervous system myelin formation
        double-stranded DNA binding
        DNA bending activity
        cell differentiation in hindbrain
        response to interleukin-6
        promoter binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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