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  • Recombinant Human Follistatin
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP176E, FNdatasheet pdfdatasheet pdf20 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Follistatin (FST) is a single-chain glycosylated secreted protein of gonadal origin, originally identified as an antagonist of activin and suppressor of follicle stimulating hormone (FSH) synthesis/secretion. FST is a key regulator in development and regeneration processes, through interaction with members of the transforming growth factor beta (TGF-beta) superfamily, such as bone morphogenetic proteins (BMPs) -2, -4 and -7, myostatin and growth differentiation factor 9 (GDF-9).

      This product's activity is measured by by its ability to neutralise the inhibitory effect of Activin A on murine MPC-11 cells. It has a typical ED50 of 0.1 to 0.4 ug/ml in the presence of 7.5ng/ml Activin A.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 200ul distilled water.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified recombinant follistatin expressed in E. coli.
    • Preservative Stabilisers
      None present
    • Activity
      Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED50 is 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A.
    • Purity
      >95% by SDS PAGE/HPLC analysis
    • Approx. Protein Concentrations
      Total protein concentration 0.1 mg/ml after reconstitution
    • Molecular Weight
      31.5kD (288 amino acid residues)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      signal transducer activity
      negative regulation of transcription from RNA polymerase II promoter
      activin binding
      negative regulation of activin receptor signaling pathway
      extracellular region
      protein binding
      hemopoietic progenitor cell differentiation
      positive regulation of hair follicle development
      negative regulation of follicle-stimulating hormone secretion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays0.10.4ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      PHP176 may be used as a standard in ELISA applications with AHP1280
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Follistatin Formats

    Formats Applications Sizes available
    Follistatin : Rec. Protein E, FN 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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