Follistatin (FST) is a single-chain glycosylated secreted protein of gonadal origin, originally identified as an antagonist of activin and suppressor of follicle stimulating hormone (FSH) synthesis/secretion. FST is a key regulator in development and regeneration processes, through interaction with members of the transforming growth factor beta (TGF-beta) superfamily, such as bone morphogenetic proteins (BMPs) -2, -4 and -7, myostatin and growth differentiation factor 9 (GDF-9).
This product's activity is measured by by its ability to neutralise the inhibitory effect of Activin A on murine MPC-11 cells. It has a typical ED50 of 0.1 to 0.4 ug/ml in the presence of 7.5ng/ml Activin A.
Purified recombinant protein - lyophilised
Reconstitute with 200ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant follistatin expressed in E. coli.
Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED50 is 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A.
>95% by SDS PAGE/HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution
31.5kD (288 amino acid residues)
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
PHP176 may be used as a standard in ELISA applications with AHP1280