Follistatin 288 Antibody | 17/2
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Enlarge- Mouse anti Human Follistatin 288
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Product TypeMonoclonal Antibody
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Clone17/2
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IsotypeIgG1
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti Human Follistatin 288 antibody, clone 17/2 recognises human follistatin isoform 288 (FS288).
Follistatin (FST) is a single-chain glycosylated protein of gonadal origin, originally identified as an antagonist of activin and suppressor of follicle stimulating hormone (FSH) synthesis/secretion. Alternative splicing of FST mRNA generates the two isoforms FS315 and FS288, the latter of which is the main cell-surface form and binds with high affinity to surface heparan sulphate proteoglycans, whilst FS315 binds with only low-affinity, and is considered to be the main circulating form of follistatin.
As well as activin, FST interacts with other growth factors such as bone morphogenetic proteins (BMPs) -2, -4 and -7, myostatin, and growth differentiation factor 9 (GDF-9), playing a key role in cellular differentiation/proliferation, embryonic development and tissue repair.
Mouse anti Human Follistatin 288 antibody, clone 17/2 has been shown to exhibit 10% cross-reactivity with FS315 in an immunoassay.
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Intended Use
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Target SpeciesHuman
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Product FormPurified IgG - liquid
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Reconstitution
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PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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Preservative Stabilisers0.09% Sodium Azide (NaN3)
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ImmunogenRecombinant human FS288.
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Purity
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Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered saline
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Fusion PartnersSpleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
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StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.
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GO Termsextracellular regionsignal transducer activityactivin bindingpositive regulation of hair follicle developmentnegative regulation of follicle-stimulating hormone secretionprotein bindingnegative regulation of transcription from RNA polymerase II promoterhemopoietic progenitor cell differentiationnegative regulation of activin receptor signaling pathway
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA Immunofluorescence Western Blotting Immunohistology - Paraffin(1) (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.- Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- ELISA
- Immunohistology
- Histology Positive Control TissueTestis
- Immunofluorescence
- Western Blotting
- Instructions For Use
Additional Follistatin 288 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| Follistatin 288 Antibody : Purified | 17/2 | E, IF, P*, WB | 0.1 mg |
- Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
Recommended Negative Isotype Control
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Histar Detection System | STAR3000B | 150 Tests | C, P | STAR3000B | |
| Histar Detection System | STAR3000A | 50 Tests | C, P | STAR3000A | |
| Histar Detection System | STAR3000C | 500 Tests | C, P | STAR3000C |
Recommended Positive Controls
Histology Controls
Testis
Application Name Reference Images Immunofluorescence
References
1. Evans,L.W. et al. (1998) Development, validation and application of an ultra-sensitive two-site enzyme immunoassay for human follistatin.J Endocrinol. 156 (2): 275-82.
2. McPherson, S.J. et al. (1999) Expression of activin A and follistatin core proteins by human prostate tumor cell lines.Endocrinology. 140 (11): 5303-9.
3. Majdic, G. et al. (1997) Testicular expression of inhibin and activin subunits and follistatin in the rat and human fetus and neonate and during postnatal development in the rat.Endocrinology. 138 (5): 2136-47.
4. Fitzgerald, A.M. et al. (2012) The effects of transforming growth factor-ß2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues.Invest Ophthalmol Vis Sci. 53 (11): 7358-69.
