Flt3 Ligand

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  • Recombinant Human Flt3 Ligand
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP085FNdatasheet pdfdatasheet pdf10 µg
    PHP085
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Human Flt3 Ligandis an E. coliderived polypeptide corresponding to the human Flt3 ligand, a soluble polypeptide comprising the extracellular domain of the transmembrane flt-3 ligand protein.Flt-3 ligand stimulates the proliferation and colony formation of certain BM precursor cells, including CD34 +ve cells. The biological activity was determined by dose-dependent stimulation of the proliferation of human AML5 cells giving an ED50 of 1.0ng/ml.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein expressed in E. coli
    • Preservative Stabilisers
      None present
    • Activity
      1 x 106 units/mg
    • Purity
      >98% by SDS PAGE and HPLC
    • Approx. Protein Concentrations
      0.1 mg/ml
    • Protein Molecular Weight
      17.6kDa (155 Amino acid sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      soluble fraction
      cytokine activity
      integral to membrane
      extracellular space
      positive regulation of cell proliferation
      plasma membrane
      signal transduction
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Flt3 Ligand Formats

    Formats Applications Sizes available
    Flt3 Ligand : Rec. Protein FN 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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