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FLIP Gamma/Delta Antibody

FLIP Gamma/Delta Antibody gallery image 1

Western blot analysis of total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K0) and NIH/3T3 cells probed with Rabbit anti FLIP gamma/delta (AHP1591)

FLIP Gamma/Delta Antibody gallery image 2

Immunocytochemical staining of HeLa cells with Rabbit anti FLIP gamma/ delta (AHP1591)

  • FLIP Gamma/Delta Antibody thumbnail image 1
  • FLIP Gamma/Delta Antibody thumbnail image 2
  • Rabbit anti FLIP Gamma/Delta (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1591C, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti FLIP Gamma/Delta antibody recognises the apoptosis related protein designated FLIP (FLICE inhibitory protein).

      FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1.

      FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. Rabbit anti FLIP Gamma/Delta antibody recognizes human and murine delta and gamma variants but not the alpha or beta, variants.

      FLIP is widely expressed in human tissues and a diverse range of cell lines.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human FLIP gamma/delta (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
    • Immunogen
      A peptide corresponding to aa191-209 at the C-terminus of human FLIP delta/FLIPs form.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen2ug/ml
      Western Blotting1/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1591 detects bands of approximately 35kDa and 25kDa in Jurkat cell lysates.
    • Instructions For Use

    Additional FLIP Gamma/Delta Antibody Formats

    Formats Applications Sizes available
    FLIP Gamma/Delta Antibody : Purified C, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • FLIP Gamma/Delta Antibody thumbnail image 1
                • FLIP Gamma/Delta Antibody thumbnail image 2

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