FKBP1A Antibody (PrecisionAbTM Antibody)

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FKBP1A Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with FKBP1A antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 41 second exposure. Arrow points to FKBP1A (molecular weight 16 kDa)

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  • FKBP1A Antibody with Control Lysate
  • Rabbit anti FKBP1A
  • FKBP1A Antibody with Control Lysate
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    Rabbit anti-human FKBP1A antibody recognizes peptidyl-prolyl cis-trans isomerase FKBP1A, also known as 12 kDa FK506-binding protein, calstabin 1, immunophilin FKBP12, protein kinase C inhibitor 2 or rotamase. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00601WBdatasheet pdfdatasheet pdf100 µl
      VPA00601
      VPA00601KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00601K
      VPA00601KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00601KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human FKBP1A antibody recognizes the peptidyl-prolyl cis-trans isomerase FKBP1A, also known as 12 kDa FK506-binding protein, 12 kDa FKBP, FKBP12-Exip3, PPIase FKBP1A, calstabin 1, immunophilin FKBP12, protein kinase C inhibitor 2 or rotamase.

        The protein encoded by the FKBP1A gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to FKBP1A, at least one of which is transcribed (provided by RefSeq, Sep 2008).

        Rabbit anti Human FKBP1A gene antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti FKBP1A detects a band of approximately 16 kDa in Raji cell lysates
        Anti FKBP1A detects a band of approximately 16 kDa in Raji cell lysates
        Anti FKBP1A detects a band of approximately 16 kDa in Raji cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
        Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        2% Sucrose
        0.09% Sodium Azide (NaN3)
        2% Sucrose
        0.09% Sodium Azide (NaN3)
        2% Sucrose
      • Immunogen
        Synthetic peptide directed towards the middle region of human FKBP1A
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Raji lysate lyophilized in RIPA buffer.
        400μg Raji lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        SMAD binding
        negative regulation of protein phosphatase type 2B activity
        FK506 binding
        protein maturation by protein folding
        protein refolding
        signal transducer activity
        type I transforming growth factor beta receptor binding
        positive regulation of protein ubiquitination
        T cell activation
        regulation of ryanodine-sensitive calcium-release channel activity
        cytosol
        regulation of activin receptor signaling pathway
        positive regulation of protein binding
        terminal cisterna
        regulation of immune response
        activin binding
        'de novo' protein folding
        positive regulation of I-kappaB kinase/NF-kappaB cascade
        heart trabecula formation
        peptidyl-prolyl cis-trans isomerase activity
        SMAD protein complex assembly
        fibril organization
        ventricular cardiac muscle tissue morphogenesis
        beta-amyloid formation
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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