Fibroblasts/Epithelial Cells Antibody | D7-FIB

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Fibroblasts/Epithelial Cells Antibody | D7-FIB gallery image 1

Staining of KG1 cells with Mouse anti Human Fibroblasts/ Epithelial cells (MCA1399G)

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Fibroblasts/Epithelial Cells Antibody | D7-FIB gallery image 2

Published customer image:
Mouse anti Human Fibroblasts/epithelial cells antibody, clone JB1B used for the detection of CD29 expressing cells in porcine meninges by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of the porcine meninges. The meninges composed of the dura mater (A–G), the arachnoid mater (H–I) and the pia mater (J–M) in formalin fixed tissue. The porcine spinal cord was cryosectioned and the porcine dura mater (N–O) was stained. The tissue sections were labeled for: (A) von Willebrand factor, (B and K) fibronectin, (C and J) vimentin, (D) laminin, (E) collagen I, (F and M) collagen II, (G, H, and L) integrin 1ß, (I) Desmoplakin, (N) collagen III, (O) E-cadherin. Images were captured at ×200 magnification.

From: Papageorgiou I, Marsh R, Tipper JL, Hall RM, Fisher J, Ingham E. Interaction of micron and nano-sized particles with cells of the dura mater. J Biomed Mater Res B Appl Biomater. 2014 Oct;102(7):1496-505.

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  • Fibroblasts/Epithelial Cells Antibody | D7-FIB thumbnail image 1
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  • Mouse anti Human Fibroblasts/Epithelial Cells:RPE
  • Mouse anti Human Fibroblasts/Epithelial Cells
  • Mouse anti Human Fibroblasts/Epithelial Cells:RPE
  • Mouse anti Human Fibroblasts/Epithelial Cells
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  • Product Type
    Monoclonal Antibody
  • Clone
    D7-FIB
  • Isotype
    IgG2a
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1399GC *, F, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA1399G
    MCA1399PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1399PE
    MCA1399GTC *, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA1399GT
    MCA1399PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1399PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB recognizes a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.

      In immunohistological studies Mouse anti Human Fibroblasts/Epithelial Cells antibody.

      Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.

      Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001).

      Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratno
      Mouseno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1.0ml distilled water.
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1.0ml distilled water.
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09%Sodium Azide
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09%Sodium Azide
    • Immunogen
      Human foreskin fibroblasts.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
    • Storage
      Store at +4oC. DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/100
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)1/100
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Fibroblasts/Epithelial Cells Antibody Formats

    Formats Clone Applications Sizes available
    Fibroblasts/Epithelial Cells Antibody : Purified D7-FIB C *, F, IP 25 µg | 0.2 mg
    Fibroblasts/Epithelial Cells Antibody : RPE D7-FIB F 25 Tests | 100 Tests
    • Copyright © 2016 Bio-Rad

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              References

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