Fibrillin 1 Antibody | 3H6

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Fibrillin 1 Antibody | 3H6 gallery image 1

Staining of paraffin embedded human kidney with Mouse anti Human FIBRILLIN 1:Azide Free (MCA3106Z)

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  • Fibrillin 1 Antibody | 3H6 thumbnail image 1
  • Mouse anti Human Fibrillin 1:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    3H6
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3106ZP *datasheet pdfdatasheet pdf0.1 mg
    MCA3106Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Fibrillin antibody, clone 3H6 recognizes human Fibrillin-1, also known as FBN1. Fibrillin is a 2844 amino acid ~300 kDa extracellular matrix protein and major component of extracellular calcium-binding microfibrils.

      Mutations in the FBN1 gene has been implicated in the manifestation of Marfan syndrome (MFS) (Dietz et al. 1991)
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 2772-2872 of human Fibrillin-1.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      microfibril
      basement membrane
      protein binding
      extracellular space
      skeletal system development
      extracellular matrix structural constituent
      negative regulation of transforming growth factor beta receptor signaling pathway by extracellular sequestering of TGFbeta
      heart development
      calcium ion binding
      negative regulation of BMP signaling pathway by extracellular sequestering of BMP
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)0.110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Fibrillin 1 Antibody Formats

    Formats Clone Applications Sizes available
    Fibrillin 1 Antibody : Preservative Free 3H6 P * 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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