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FHL1 Antibody

FHL1 Antibody gallery image 1

Human muscle lysate probed with Goat anti human FHL1 (AHP2070)

FHL1 Antibody gallery image 2

Paraffin embedded human testis stained with Goat anti human FHL1 (AHP2070)

  • FHL1 Antibody thumbnail image 1
  • FHL1 Antibody thumbnail image 2
  • Goat anti Human FHL1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2070E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human FHL1 antibody recognizes human Four and a half LIM domains protein 1, also known as FHL1, Skeletal muscle LIM-protein 1 or SLIM-1. FHL1 is a 323 amino acid ~30 kDa cytoplasmic protein containing multiple LIM domains. FHL1 is abundantly expressed by skeletal muscle and has been linked with several muscular dystrophy-like muscle disorders, including Scapuloperoneal syndrome (Quinzii et al. 2008), X-linked severe early-onset reducing body myopathy (Schlessl et al. 2011), and X-linked myopathy with postural muscle atrophy (Windpassinger et al. 2008).

      Goat anti Human FHL1 antibody is expected to recognise FHL1 isoform 5 and isoform 2, the latter being implicated as a negative regulator of RBP J- mediated transcription (Liang et al. 2008).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      DogExpected from Sequence
      RatExpected from Sequence
      BovineExpected from Sequence
      PigExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human FHL1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence CNKRFVFHNEQVY from the internal region of FHL1 (NP_001440.1; NP_001153171.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.050.3ug/ml
      Immunohistology - Paraffin(1)2.02.5ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human testis.
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional FHL1 Antibody Formats

    Formats Applications Sizes available
    FHL1 Antibody : Purified E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human testis.
                • Product Images

                • FHL1 Antibody thumbnail image 1
                • FHL1 Antibody thumbnail image 2


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