FGF Acidic

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  • Recombinant Human FGF Acidic
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP031FNdatasheet pdfdatasheet pdf50 µg
    PHP031
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human FGF acidic, also known as FGF 1 (fibroblast growth factor 1), is a member of the heparin-binding growth factor family, widely distributed in the peripheral and central nervous systems.

      FGF 1 acts as a potent neurotrophic factor, playing a key role during prenatal development and postnatal growth/regeneration and is dependent upon interactions with heparin sulfate (HS) proteoglycans located on cell surfaces and in the extracellular matrix, and the four high affinity tyrosine kinase receptors, designated FGFR-1,-2,-3 and -4.

      Bioactivity was determined by the dose-dependent stimulation of thymidine uptake by FGF receptor-expressing BaF3 cells. The ED50 is <10ng/ml.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified - lyophilised
    • Reconstitution
      Reconstitute with 50ul of 5mM Sodium Phosphate, pH 8.0. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
    • Preparation
      Recombinant protein expressed in E. coli.
    • Preservative Stabilisers
      None present
    • Activity
      1 x 105 units/mg
    • Purity
      >95% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      1 mg/ml after reconstitution
    • Protein Molecular Weight
      15.8 kDa (140 Amino Acid Sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      angiogenesis
      growth factor activity
      positive regulation of cholesterol biosynthetic process
      insulin receptor signaling pathway
      heparin binding
      S100 alpha binding
      positive regulation of cell division
      cytosol
      positive regulation of intracellular protein kinase cascade
      fibroblast growth factor receptor signaling pathway
      fibroblast growth factor receptor binding
      positive regulation of gene-specific transcription from RNA polymerase II promoter
      cellular response to heat
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional FGF Acidic Formats

    Formats Applications Sizes available
    FGF Acidic : Rec. Protein FN 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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