FGF 4 (fibroblast growth factor 4) is a member of the heparin-binding growth factors family which act as promoters of cellular differentiation and proliferation, and therefore play key roles in embryonic development, morphogenesis, tissue regeneration and tumour growth and invasion. Studies have shown that FGF 4 signals through binding to the FGF Receptors (FGFRs) 1c, 2c, 3c, and 4.
Purified recombinant protein - lyophilised
Reconstitute with 250ul distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant FGF 4 expressed in E.coli
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is = 0.5 ng/ml, corresponding to a specific activity of = 2 x 106 units/mg.
>95% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
19.7kD (182 amino acid residues)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.