FEN1 Antibody

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Detection of human Fen1 in whole cell lysates of HeLa and 293T cells (5-50 mcg/lane) by Western blotting with Rabbit anti Human Fen1 (AHP837). Immunoprecipitation was with AHP837 (3mcg/mg lysate) or another anti Fen1 polyclonal antibody, subsequent detection of immunoprecipitated Fen1 by Western blotting used Rabbit anti Fen-1 antibody (AHP837)

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  • Rabbit anti Human FEN1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP837IP, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP837
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    References
    Reviews
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    • Rabbit anti Human FEN1 antibody recognizes human Flap endonuclease 1 also known as Flap structure-specific endonuclease, FEN1, Maturation factor 1 or DNase IV. FEN1 is a 380 amino acid ~45kDa member of the FEN1 sub family of XPG/RAD2 endonucleases which has an important role in lagging strand DNA synthesis and base excision repair (Kim et al. 1998). It is hypothesised that FEN1 may play a role in the inhibltion of triplet repeat expansion (Vallur and Maizels 2010).

      Two isoforms of FEN1 are generated by alternative initiation, isoform 1 against which rabbit anti human FEN1 antibody is generated and a truncated isoform FENMIT, lacking the N-terminal region and having a mitochondrial distribution, FENMIT also lacks DNAase activity (Kazak et al. 2013). Initial results suggest that rabbit anti human FEN1 antibody does not recognize the truncated form of human FEN1.

      Fen1 is expressed in the nucleus of a wide variety of organ strusctures notably in those of the hematopoietic system including spleen, lymph nodes and spleen. Rabbit anti human FEN1 antibody recognizes a band of ~45kDa in whole cell lysates of HeLa and 293T by western blotting and has successfully been used for immunoprecipitation of FEN1 from HeLa cell lysates.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation2ug/mg protein10ug/mg protein
      Western Blotting1/20001/10000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP837 detects a band of approximately 42kD in HeLa cell lysates.
    • Instructions For Use

    Additional FEN1 Antibody Formats

    Formats Applications Sizes available
    FEN1 Antibody : Purified IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

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          Recommended Positive Controls

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