Factor VIII Antibody | RFF-VIIIC/8

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Factor VIII Antibody | RFF-VIIIC/8 gallery image 1

Recombinant Factor VIII detected with Mouse anti Human Factor VIII (MCA4677)

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Image caption:
Specificity of FVIII activity assay is demonstrated by antibody inhibition and sample dilutions. (A) FVIII activities, ranging from 0.6–10 mU/ml, in dilutions of rFVIII, were inhibited by addition of 10 μg/ml mouse anti-human FVIII antibody (clone RFF-VIIIC/8) for 10 min (on ice) prior to the start of the chromogenic Coatest assay. The green triangles represent rFVIII without antibody addition and the red squares and blue diamonds are 2 separate rFVIII dilutions with final concentrations of 10 µg/ml anti-FVIII. (B) FVIII activity was measured in HUVEC cell lysates diluted 1.3-, 2-, 3- and 4-fold in 1% BSA/PBS.

From: Turner NA, Moake JL (2015) Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings.
PLoS ONE 10(10): e0140740.

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  • Mouse anti Human Factor VIII
  • Mouse anti Human Factor VIII
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    RFF-VIIIC/8
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4677GE, R, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA4677G
    MCA4677E, R, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA4677
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 recognizes human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream.

      Defects in Factor VIII cause haemophilia A, a disorder characterised by the body’s inability to control blood clotting. This could result in severe blood loss, even with minor injuries.

      Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 is a very potent coagulation inhibitor. It recognizes an epitope towards the N-terminus of full-length Factor VIII. It also recognizes the 210kDa, 90kDa and 40kDa cleavage products. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 does not cross-react with von Willebrand factor.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      Mouseno
      Dogno
      Ratno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.5 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.5 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Affinity purified human Factor VIII.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunized Balb/c mouse we fused foth cells of the P3-NS/a-Ag4-1 mouse myeloma.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      copper ion binding
      extracellular space
      platelet alpha granule lumen
      cell adhesion
      protein binding
      acute-phase response
      oxidoreductase activity
      platelet degranulation
      plasma membrane
      platelet activation
      blood coagulation, intrinsic pathway
      oxidation-reduction process
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/15000
      Radioimmunoassays
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/15000
      Radioimmunoassays
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Factor VIII Antibody Formats

    Formats Clone Applications Sizes available
    Factor VIII Antibody : Purified RFF-VIIIC/8 E, R, WB 0.2 mg | 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISA

              References

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