Ezrin (pTyr353) Antibody

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Ezrin (pTyr353) Antibody gallery image 1

Western blot analysis of extracts from HEK-293 cells untreated or treated with EGF using Rabbit anti ezrin (pTyr353) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes

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Ezrin (pTyr353) Antibody gallery image 2

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti ezrin (pTyr353) antibody

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  • Rabbit anti Ezrin (pTyr353)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2613P, WBdatasheet pdfdatasheet pdf50 µl
    AHP2613
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti ezrin (pTyr353) antibody recognizes ezrin, also known as villin-2, when phosphorylated at tyrosine 353. Ezrin is a member of the Ezrin Radixin Mosin (ERM) family. Ezrin functions as a linker molecule between the plasma membrane and the cytoskeleton. Phosphorylation of ezrin at tyrosine 353 occurs following treatment of cells with epidermal growth factor. Phosphorylation of ezrin at tyrosine 353 is related to signal survival of PI3K/Akt pathway (Gautreau et al., 1999)
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/501/100
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional Ezrin (pTyr353) Antibody Formats

    Formats Applications Sizes available
    Ezrin (pTyr353) Antibody : Purified P, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

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          Recommended Positive Controls

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