EB07305 (1µg/ml) staining Western blotting analysis of Human Placenta lysate (35μg protein in RIPA buffer) probed using Goat anti Human ezrin antibody (AHP1494) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Goat anti Human Ezrin antibody recognizes human ezrin, otherwise known as p81 or villin 2, Ezrin is a 586 amino acid ~80kDa cytoplasmic peripheral membrane protein containing a single FERM domain and member of the ezrin, radixin, moesin (ERM) protein family, which acts as protein-tyrosine kinase substrate.
Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization (Ng et al. 2001). Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44 (Finnerty et al. 2004). Studies have implicated ezrin in the metastatic capacity of various types of human tumours including breast, prostate, bone, endometrial and colon cancers (Ohtani et al. 1999, Elzaqheid et al. 2008).
Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antiserum to human ezrin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Peptide sequence C-QDYEEKTKKAER from the internal region of ezrin (NP_003370.2).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1394 detects a band of approximately 80kDa in human placenta and duodenum cell lysates.