Estrogen Receptor Beta 5 Antibody | 5/25

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Estrogen Receptor Beta 5 Antibody | 5/25 gallery image 1

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta5 antibody (MCA4676) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power)

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Estrogen Receptor Beta 5 Antibody | 5/25 gallery image 2

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta5 antibody (MCA4676) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power)

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Estrogen Receptor Beta 5 Antibody | 5/25 gallery image 3

Published customer image:
Mouse anti Human estrogen receptor β5 antibody, clone 5/25 used for the detection of ERβ5 expressing cells in human endometrial cancer tissue biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. All proteins were immunolocalised to cell nuclei (see higher power inserts in panels e, f, c and d respectively). In the well and moderately differentiated cancers expression was most intense in epithelial cell layers (arrowheads, panel A and inserts). Note that ERa was low/absent in poor grade cancers (J) but immunoexpression of ERß1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERß1, ERß2 and ERß5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers.

From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330.

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Estrogen Receptor Beta 5 Antibody | 5/25 gallery image 4

Published customer image:
Mouse anti Human estrogen receptor β5 antibody, clone 5/25 used for the detrction of ERβ5 expressing cells in tumors by immunohistochemistry formalin fixed, paraffin embedded tissue sections.
Image caption:
Effect of gender on ERß1, ERß2 and ERß5 expression in D283 Med xenografts. A) Immunostaining for ERß1 was significantly higher in tumors from females compared to males (**p<0.01); B) and C) Tumor levels of ERß2 and ERß5 did not significantly differ between females and males. All results are expressed as the mean ± SEM (n = 6 tumors/group). Representative stained section of tumors from females and males are shown (magnification 40x). IHC, immunohistochemical score.

From:Ciucci A, Meco D, De Stefano I, Travaglia D, Zannoni GF, et al. (2014) Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor ß Signaling Play a Role? PLoS ONE 9(7): e101623.

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Estrogen Receptor Beta 5 Antibody | 5/25 gallery image 5

Published customer image:
Mouse anti Human estrogen receptor β5 antibody, clone 5/25 used for the detrction of ERβ5 expressing cells in normal human liver by immunohistochemistry formalin fixed, paraffin embedded tissue sections.
Image caption:
Staining pattern of the ERß isoforms in human liver. In order to confirm the specificity of the ERß antibodies used in the study, we immunostained sections of human healthy liver, showing that ERß1 and ERß2 isoforms are not expressed, while specific nuclear ERß5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ERß isoforms immunoreactivity is consistent with previous descriptions.

From:Ciucci A, Meco D, De Stefano I, Travaglia D, Zannoni GF, et al. (2014) Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor ß Signaling Play a Role? PLoS ONE 9(7): e101623.

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  • Mouse anti Human Estrogen Receptor Beta 5
  • Mouse anti Human Estrogen Receptor Beta 5
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5/25
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4676GAP *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4676GA
    MCA4676TP *, WBdatasheet pdfdatasheet pdf25 µg
    MCA4676T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti human estrogen receptor beta 5, clone 5/25 specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5) (Moore et al. 1998). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription (Shaaban et al. 2008).

      Immunohistochemical studies have revealed a ubiquitous expression of ERB5 in normal colorectal tissue, and to be present in all primary colorectal carcinomas studied (Wong, N. et al. 2005).

      The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy transcription (Shaaban et al. 2008, Collins et al. 2009).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Tuberculin conjugated synthetic peptide LLSHVRHARYAP derived from the C-terminus of human ERB5.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Normal colon or colon carcinoma
    • Histology Positive Control Tissue
      Normal colon or colon carcinoma
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Estrogen Receptor Beta 5 Antibody Formats

    Formats Clone Applications Sizes available
    Estrogen Receptor Beta 5 Antibody : Purified 5/25 P *, WB 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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          Recommended Positive Controls

            Histology Controls

              Normal colon or colon carcinoma
              Normal colon or colon carcinoma
              • Application NameReference Images
                Immunohistology - Paraffin
                Western Blotting

              References

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