Estrogen Receptor Alpha (pSer106) Antibody

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Estrogen Receptor Alpha (pSer106) Antibody gallery image 1

Western blot analysis of extracts from MCF-7 cells untreated or treated with EGF using Rabbit anti estrogen receptor alpha (pSer106) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes

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Estrogen Receptor Alpha (pSer106) Antibody gallery image 2

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti estrogen receptor alpha (pSer106) antibody

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  • Rabbit anti Estrogen Receptor Alpha (pSer106)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2610P, WBdatasheet pdfdatasheet pdf50 µl
    AHP2610
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti estrogen receptor alpha (pSer106) antibody recognizes estrogen receptor alpha, also known as ER-alpha, when phosphorylated at serine 106. Estrogen receptor alpha is a member of the nuclear receptor superfamily and also functions as a ligand-regulated transcription factor. Estrogen receptor alpha consists of several domains including the transcription activation function-1 domain (AF-1), the DNA-binding domain, the hinge domain and the ligand-binding domain (AF-2). Estrogen receptor alpha is phosphorylated at serine 106 by MAPKs, Cdk2 and GSK3 (Thomas et al. 2008, Rogatsky et al. 1999, Medunjanin et al. 2005). Phosphorylation at serine 106 contributes to agonist independent activation of estrogen receptor alpha.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to estrogen receptor alpha (pSer106) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
      50% Glycerol
    • Immunogen
      Phospho specific-peptide corresponding to residues surrounding serine 106 of human estrogen receptor alpha
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      nucleoplasm
      cytoplasm
      beta-catenin binding
      chromatin remodeling complex
      regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
      estrogen receptor activity
      enzyme binding
      response to estradiol stimulus
      nitric-oxide synthase regulator activity
      sequence-specific DNA binding transcription factor activity
      estrogen response element binding
      zinc ion binding
      steroid binding
      positive regulation of retinoic acid receptor signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/501/100
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional Estrogen Receptor Alpha (pSer106) Antibody Formats

    Formats Applications Sizes available
    Estrogen Receptor Alpha (pSer106) Antibody : Purified P, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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        Useful Reagents

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          Recommended Positive Controls

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              Further Reading

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